? ? ? ? ? 文中所述的MPRP方法是將靈敏的多重real time PCR方法與滾環(huán)式DNA擴增方法相結合，解決了很多其他基于PCR的SNP檢測方法中存在的靈敏度低、通量小、復雜的操作以及高成本等問題，同時實現了便捷地多重real time PCR檢測。文中將MPRP方法與三通道微滴芯片式數字PCR平臺結果進行比較，充分表明該方法在對靶向藥物的患者篩選和耐藥性預測中具備顯著的應用前景。

數字PCR測定的2D圖 ↑

紅圈中的點數表示陽性微滴的數量

（A）患者P6的EGFR L858R測定的數字PCR結果

（B）患者P1的EGFR T790M測定的數字PCR結果

? ? ? ? ? 隨著靶向藥物的持續(xù)開發(fā)和應用，需建立非入侵性診斷方法以便篩選可進行治療的患者。而且多個腫瘤相關突變檢測對于治療和耐藥性預測意義重大。雖然有很多檢測SNP的PCR方法，但是由于靈敏度低、通量小、復雜的操作以及高成本等問題，應用嚴重受限。為解決上述問題，本文描述的MPRP方法，將靈敏的多重real time PCR方法與滾環(huán)式DNA擴增方法進行了有效結合。提高了SNP檢測的特異性和靈敏度，同時實現便捷地多重real time PCR檢測。在8位患者樣本的MPRP方法與數字PCR方法的檢測結果比較中，充分表明MPRP方法具有潛在的臨床應用前景。同時可以看出，MPRP方法是滿足腫瘤相關突變多重檢測的特異性和靈敏度要求且便捷的方法。

With the continuous development and application of targeted drugs, it is particularly desirable to ?nd a non- invasive diagnostic approach to screen patients for precision treatment. Speci?cally, detection of multiple cancer-related mutations is very important for targeted therapy and prediction of drug resistance. Although numerous advanced PCR methods have been developed to discriminate single nucleotide polymorphisms, their drawbacks signi?cantly limit their application, such as low sensitivity and throughput, complicated operations, and expensive costs. In order to overcome these challenges, in this study, we? developed a method combining multiplex and sensitive real-time PCR assay with rolling circle ampli?cation. This allows speci?c and sensitive discrimination of the single nucleotide mutation and provides convenient multiplex detection by real-time PCR assay. The clinical potential of the MPRP assay was further demonstrated by comparing samples from 8 patients with a digital PCR assay. The coincident results between these two methods indicated that the MPRP assay can provide a speci?c, sensitive, and convenient method for multiplex detection of cancer-related mutations.